国产综合一区二区三区,99香蕉国产精品偷在线观看,性色少妇AV蜜臀人妻无码,成版人app网站

您好,歡迎進(jìn)入上海力敏實(shí)業(yè)有限公司網(wǎng)站!
一鍵分享網(wǎng)站到:
您現(xiàn)在的位置:首頁 >> 產(chǎn)品中心 >> 生化試劑、抗體、血清 >> 抗體/蛋白 >> SC-2002santacruz G蛋白PLUS-瓊脂糖Protein G PLUS-Agarose

santacruz G蛋白PLUS-瓊脂糖Protein G PLUS-Agarose

  • 更新時(shí)間:  2023-07-25
  • 產(chǎn)品型號(hào):  SC-2002
  • 簡單描述
  • Protein G PLUS-Agarose is suitable for immunoprecipitation of mouse IgG1,
    IgG2a, IgG2b and IgG3, rat IgG1, IgG2a, santacruz G蛋白PLUS-瓊脂糖Protein G PLUS-AgaroseIgG2b and IgG2c, rabbit and goat polycl
詳細(xì)介紹

santacruz  G蛋白PLUS-瓊脂糖Protein G PLUS-Agarose

SANTA CRUZ BIOTECHNOLOGY, INC.
Protein G PLUS-Agarose
Immunoprecipitation Reagent: sc-2002
Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com
PRODUCT
Protein G PLUS is provided as an agarose conjugate for use in immunoprecipitation
only. The product is provided as 0.5 ml agarose in 2.0 ml PBS buffer
with 0.02% azide. Protein G PLUS-Agarose is pre-blocked with BSA to reduce
non-specific immunoglobulin binding. Sufficient product is provided for 100
immunoprecipitation reactions, to be used at 20 μl resuspended volume per
reaction.
SPECIFICITY
Protein G PLUS-Agarose is suitable for immunoprecipitation of mouse IgG1,
IgG2a, IgG2b and IgG3, rat IgG1, IgG2a, IgG2b and IgG2c, rabbit and goat polyclonal
Abs, and human IgG1, IgG2, IgG3 and IgG4.
PROCEDURE
? Incubate cultured cells (80-90% confluent monolayer in 100 mm cell culture
plate, or approximay 2-5 x 107 suspension cells in flask) in methioninefree
medium containing 5% dialyzed fetal calf serum for 1 hour at 37° C.
The same procedure can be used for cells labeled with other radioactive
amino acids (e.g., 14C or 3H) or with γ32P-orthophosphate. Cell labeling must
be carried out in medium lacking the relevant amino acid or in phosphatefree
medium.
? Remove medium and replace with 3 ml methionine-free medium containing
5% dialyzed fetal calf serum and 100 μCi/ml 35S-methionine. Incubate 1
hour at 37° C. For some proteins a longer labeling period (up to 18 hours)
is preferable.
? Carefully remove radioactive medium with Pasteur pipette and wash cell
monolayer with PBS.
? Add 3 ml ice cold RIPA buffer to cell monolayer and incubate at 4° C for
10 minutes. For suspension cells, add the RIPA buffer to washed cell pellet
in a 15 ml conical centrifuge tube.
? Disrupt cells by repeated aspiration through a 21 gauge needle and transfer
to a 15 ml conical centrifuge tube.
? Wash cell culture plate with additional 1.0 ml ice cold RIPA buffer and
combine with original extract.
? Pellet cellular debris by centrifugation at 10,000 xg for 10 minutes at 4° C.
Transfer supernatant to a fresh 15 ml conical centrifuge tube on ice.
Preclear lysate (optional step) by adding 1.0 μg of the appropriate control
IgG (normal mouse, rat, rabbit or goat IgG, corresponding to the host
species of the primary antibody), together with 20 μl of resuspended
volume of Protein G PLUS-Agarose. Incubate at 4° C for 30 minutes.
? Pellet beads by centrifugation at 2,500 rpm (approximay 1,000 xg) for
5 minutes at 4° C. Transfer supernatant (cell lysate) to a fresh 15 ml conical
centrifuge tube on ice.
? Transfer 1 ml of the above cell lysate, or approximay 100-500 μg total
cellular protein, to a 1.5 ml microcentrifuge tube. Add 1-10 μl (i.e., 0.2-2 μg)
primary antibody (optimal antibody concentration should be determined by
titration) and incubate for 1 hour at 4° C.
? Add 20 μl of resuspended volume of Protein G PLUS-Agarose. Cap tubes
and incubate at 4° C on a rocker platform or rotating device for 1 hour to
overnight.
? Collect immunoprecipitates by centrifugation at 2,500 rpm (approximay
1,000 xg) for 5 minutes at 4° C. Carefully aspirate and discard radioactive
supernatant.
? Wash pellet 4 times with 1.0 ml RIPA buffer (more stringent) or PBS (less
stringent), each time repeating centrifugation step above.
? After final wash, aspirate and discard supernatant and resuspend pellet in
40 μl of 1x electrophoresis sample buffer.
? Boil samples for 2–3 minutes and analyze 20 μl aliquots by SDS-PAGE and
autoradiography. Unused samples may be stored at -20° C.
? Optional: After boiling, samples may be centrifuged to pellet the agarose
beads followed by SDS-PAGE analysis of the supernatant.
SELECT PRODUCT CITATIONS
1. Medema, R., et al. 1998. p21waf1 can block cells at two points in the cell
cycle, but does not interfere with processive DNA-replication or stressactivated
kinases. Oncogene 16: 431-441.
2. Marzo, I., et al. 1998. Bax and adenine nucleotide translocator cooperate
in the mitochondrial control of apoptosis. Science 281: 2027-2031.
3. Song, Y., et al. 2011. Ligand-dependent corepressor acts as a novel corepressor
of thyroid hormone receptor and represses hepatic lipogenesis in
mice. J. Hepatol. 56: 248-254.
4. Beard, R.S., et al. 2012. Metabotropic glutamate receptor 5 mediates
phosphorylation of vascular endothelial cadherin and nuclear localization
of β-catenin in response to homocysteine. Vascul. Pharmacol. 56: 159-167.
STORAGE
Store at 4° C, do not freeze; stable for one year from the date of shipment.
RESEARCH USE
For research use only, not for use in diagnostic procedures.
Immunoprecipitation agarose conjugates are pre-blocked with BSA to reduce non-specific immunoglobulin
binding and are provided at a concentration (0.5 ml agarose/2.0 ml) suitable for use at 20 μl per
immunoprecipitation reaction. Number of reactions: 100.

 

上海力敏實(shí)業(yè)有限公司代理santacruz產(chǎn)品,咨詢santacruz  G蛋白PLUS-瓊脂糖Protein G PLUS-Agarose 詳細(xì)產(chǎn)品信息


留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
亚洲无人区乱码中文字幕| 久久久久亚洲av无码专区首| 真实国产老熟女粗口对白| 年轻老师2韩国手机在线| 51精产国品一二三产区区别 | 日韩一线无码AV毛片免费| 久久久精品波多野结衣| 五十路六十路老熟妇A片| 国产美女在线精品免费观看网址| 师娘求放过完整高清在线看| 性欧美xxxxx极品少妇| 99精品国产在热久久无码| 成人国产精品玖玖热色欲| 被老外添嫩苞添高潮np| 精品久久久久久久久久中文字幕| 国产熟女一区二区三区五月婷| 亚洲精品第一国产综合野草社区| 国产精品无码无卡无需播放器 | 国产精品一区二区毛卡片| 国产成人久久婷婷精品流白浆 | 亚洲一区二区三区高清在线观看| 中文字幕有码无码人妻AV蜜桃| 免费观看夜里30款禁用软件| 成人网站在线观看| 久久久久精品无码专区| 国产又黄又大又粗的视频| 精品人人妻人人澡人人爽牛牛| 被强迫各种姿势侵犯N| 日日摸天天爽天天爽视频| free性开放小少妇| 免费无码又色又爽的视频软件| 中文字幕AV久久一区二区| 大肉蒂被嘬的好爽h| 羞答答的玫瑰静悄悄的开歌词| 中文字幕一线产区和二线区的区别 | 国产毛a片啊久久久久久保和丸| 久久精品99国产精品日本| 一本久久A久久精品亚洲| 久久人妻少妇嫩草av无码 | 热の综合热の国产中文网| 少妇性夜夜春夜夜爽A片|