国产综合一区二区三区,99香蕉国产精品偷在线观看,性色少妇AV蜜臀人妻无码,成版人app网站

您好,歡迎進(jìn)入上海力敏實(shí)業(yè)有限公司網(wǎng)站!
一鍵分享網(wǎng)站到:
產(chǎn)品搜索
PRODUCT SEARCH
產(chǎn)品分類(lèi)
PRODUCT CLASSIFICATION
查看更多 >>
相關(guān)文章
RELEVANT ARTICLES
您現(xiàn)在的位置:首頁(yè) >> 產(chǎn)品中心 >> >> >> B-1255VECTOR 生物素莧尾狀凝集素(ACL,ACA) BIOTIN-ACL

VECTOR 生物素莧尾狀凝集素(ACL,ACA) BIOTIN-ACL

  • 更新時(shí)間:  2023-07-24
  • 產(chǎn)品型號(hào):  B-1255
  • 簡(jiǎn)單描述
  • VECTOR 生物素莧尾狀凝集素(ACL,ACA) BIOTIN-ACL
    Biotinylated Amaranthus Caudatus Lectin (ACL, ACA)
    Detection of Glycoproteins using Lectins in Histochemistry,
    ELISA, and Western Blot Applications
詳細(xì)介紹

VECTOR 生物素莧尾狀凝集素(ACL,ACA) BIOTIN-ACL

The following protocols offer guidelines for assay development using lectin-based detection of glycoproteins
present in tissue sections, adsorbed onto microtiter plates, or transferred from electrophoretic
gels onto nitrocellulose or PVDF membranes.
Histochemistry:
1a. Staining procedure for paraffin sections: Deparaffinize and hydrate tissue sections through
xylenes or other clearing agents and graded alcohol series and rinse for 5 minutes in tap water.
If required, retrieve antigens using the Antigen Unmasking Solution (H-3300 or H-3301).
1b. Staining procedure for frozen sections: Air dry sections. Immediay before staining, fix
sections with acetone. Transfer slices to buffer. If endogenous enzyme activities are present,
inactivate using appropriate methods.
2. Perform Streptavidin/Biotin blocking if required following kit instructions (SP-2002). Do not
use SP-2001. Block non-specific binding by incubating section with Carbo-Free™ Blocking
Solution (Cat. No. SP-5040) for 30 minutes at room temperature. Blot excess blocking solution
from the sections.
3. Apply biotinylated lectin at approximay 2-20 μg/ml in PBS (10 mM sodium phosphate, 150
mM NaCl, pH 7.4) to the sections and incubate for 30 minutes at room temperature. Wash with
TPBS (PBS + 0.05% Tween™20).
4. Prepare VECTASTAIN®
® ABC (peroxidase, Cat. No. PK-6100) or VECTASTAIN® ABC-AP
(alkaline phosphatase, Cat. No. AK-5000) reagents according to the kit instructions. Apply to the
sections and incubate for 30 minutes at room temperature. Wash with TPBS.
5. Apply an appropriate precipitating substrate for the enzyme system used in step 4. For peroxidase,
ImmPACT™ DAB (Cat. No. SK-4105) is recommended; for alkaline phosphatase, Vector® Red
(Cat. No. SK-5100). Rinse in tap water.
6. Counterstain (optional), clear and mount. For galactose or GalNAc-specific lectins avoid mounting
in glycerol-based mounting media.
VECTOR 生物素莧尾狀凝集素(ACL,ACA) BIOTIN-ACL

ELISA:
1. Adsorb target protein to microtiter plate by placing 50-200 μl of approximay 3 μg/ml glycoprotein
solution into the desired wells. Some wells may be left untreated as negative controls. Incubate at
37 oC for 1 hour. Wash wells three times with TPBS (PBS + 0.05% Tween™20).
2. Block non-specific binding by filling each well to the brim with Carbo-Free™ Blocking Solution
(Cat. No. SP-5040) for 30 minutes at room temperature. Wash wells three times with TPBS.
3. Apply 50-200 μl of approximay 2-20 μg/ml biotinylated lectin in PBS to the wells and incubate
for 30 minutes at room temperature. Wash wells three times with TPBS.

4. Prepare VECTASTAIN®
® ABC (peroxidase, Cat. No. PK-6100) or VECTASTAIN® ABC-AP
(alkaline phosphatase, Cat. No. AK-5000) reagents according to the kit instructions. Apply to the
wells and incubate for 30 minutes at room temperature. Wash wells three times with TPBS.
5. Apply an appropriate non-precipitating substrate for the enzyme system used in step 4. For
peroxidase, ABTS (Cat. No. SK-4500) is recommended; for alkaline phophatase, pNPP (Cat. No.
SK-5900).
6. Quantify the colored reaction product by spectrophotometry.
Western Blot:
1. Perform electrophoresis and transfer proteins to a membrane according to standard procedures.
2. Block non-specific binding by incubating the membrane in Carbo-Free™ Blocking Solution (Cat.
No. SP-5040) for 30 minutes at room temperature. Use a sufficient volume to compley cover
the membrane.
3. Incubate membrane in PBS containing approximay 2-20 μg/ml biotinylated lectin for 30 minutes
at room temperature. Wash with TPBS (PBS +0.05% Tween™20).
4. Prepare VECTASTAIN®
® ABC (peroxidase, Cat. No. PK-6100) or VECTASTAIN® ABC-AP
(alkaline phosphatase, Cat. No. AK-5000) reagents according to the kit instructions. Incubate the
membrane in the reagent for 30 minutes at room temperature. Wash with TPBS.
5. Apply an appropriate substrate for the enzyme system used in step 4. For peroxidase, DuoLuX™
Chemiluminescent/Fluorescent Substrate for Peroxidase (Cat. No. SK-6604) or ImmPACT™ DAB
(Cat. No. SK-4105) are recommended; for alkaline phosphatase, Chemiluminescent/Fluorescent
Substrate for Alkaline Phosphatase (Cat. No. SK-6605) or BCIP/NBT (Cat. No. SK-5400) are
recommended.
Negative Controls
Negative controls should be run in parallel in each of the above described methodologies to validate
binding results. When applying lectins, one of the most appropriate negative controls is to preabsorb
the lectin with a concentration of a defined sugar, with which, the lectin has a known high affinity.
Vector Labs offers a series of sugars that are intended for such a purpose.
The lectin is diluted to a suitable working concentration in a solution containing approximay
200 mM to 500 mM of the sugar. This mixture is left to bind at room temperature for 30 to 60 min.
Following this absorption incubation, the mixture is substituted into the procedure in place of the unabsorbed
lectin and incubated under the same conditions. The subsequent detection procedure is followed
as for the test method. In most cases the vast majority of lectin binding to the tissue section (membrane
blot, etc.) will be eliminated. Some trace binding to the section (blot etc) may still be present under
these conditions and probably indicates presence of secondary or tertiary sugar preferences. These negative
control results should be compared with the test results to determine specificity of binding

 

上海力敏實(shí)業(yè)有限公司代理VECTOR產(chǎn)品,咨詢(xún) 詳細(xì)產(chǎn)品信息


留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
日韩精品无码专区免费播放| 久久超碰97人人做人人爱| 日韩乱码人妻无码中文字幕| 日本护士吞精囗交gif| 色五月丁香六月欧美综合| 联系附近成熟妇女| 欧美黑人又粗又大的性格特点| 扒开双腿疯狂进出爽爽爽视频| 伊人成色综合人夜夜久久| 国产女明星裸体xxxx| 亚洲精品国产suv一区88| 免费a级毛片无码a∨免费软件| 一边摸一边叫床一边爽| 无码人妻AⅤ一区二区三区蜜桃| 久久婷婷五月综合国产| 免费久久人人爽人人爽av| 欧美白人最猛性XXXXX| 猛烈顶弄h禁欲老师h春潮| 国产女人18毛片水真多18精品| 人性短片30分钟| 无码丰满熟妇juliaann与黑人| 久久国产精品亚洲婷婷a片| 肉色欧美久久久久久久蜜桃| 国产精品一区二区在线观看| 国产精品久久人妻无码波多野结衣| 乱人伦人妻中文字幕无码| 国产精品一线二线三线有什么区别 | 国产做A爱片久久毛片A片| chinese国产xxx实拍| 高洁在公车被灌满jing液| 国产精品一国产精品| 柔术美女全婐体一丝不一挂| 欧美老肥婆牲交videos| 放荡的小峓子2中文字幕| 亚洲色无码a片一区二区情欲| 明星的发泄室nph| 国产乡下妇女做爰| 狠狠色噜噜狠狠狠狠av不卡| 杨门十二寡妇肉床艳史电影| 天堂www中文在线资源| 久久久无码一区二区三区|